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For some languages, you will hear the translation spoken aloud. They both use their own cognitive services to translate spoken words and phrases into a language of your choice. Nepali speech translation service is provided by both Microsoft and Google. Whenever you type any letter, you will get additional hints to make your typing easier. Online nepali unicode tool will automatically convert any roman text into unicode nepali. ![]() This nepali typing is absolutely free and you can email the text you have typed to anyone - including yourself. Typing nepali is natural and you don’t need to remember complex nepali keyboard. typing "Tapai lai kasto chha" gives you "तपाई लाई कस्तो छ". With this tool you can type in English and Get in nepali. Finally, don't forget to give us a like and share it on Facebook with your loved one. If you have any suggestions, and the translated sentence is way too funny then please share with us on our Facebook page. You can copy the translated text and then share them either on social media such as Facebook, Twitter or email it to your friends or family. For these purposes, this tool can be used. Offline language translator with voice professional#While it is a good idea to pay for translating lots of text (such as books, articles) and for professional service, there is no point paying for commonly used sentences, greeting messages, and other informal use. Many websites provide services to translate Nepali for a few dollars. For the rest who cannot speak the Nepali Language, translating Nepali to English could be quite difficult. Click on it, then open the Translate menu from the top right menu to select the language (s) you want to. Once the subtitle has been processed, it will be added as a new track. More than 30 million people around the world speak this language. Click the video’s track on the timeline and select the Generate Auto Subtitle tool from the Audio menu on the right side of the screen. Hopefully, one day it will produce near to perfect translation! Offline language translator with voice software#This translation software is evolving day by day and Google EngineersĪre working on it to make Nepali translation more intelligent and accurate. Although this translation is not 100% accurate, you can get a basic idea and with few modifications, it can be pretty accurate. The translation only takes a few seconds and allows up to 500 characters to be translated in one request. Our app then translates your English word, phrase, or sentence into Nepali. You can start typing on the left-hand text area and then click on the "Translate" button. Our English to Nepali Translation Tool is powered by Google Translation API. ![]()
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![]() ![]() 24 Aggies go to Wells to earn win over SF Austin. After the game, Globetrotter stars will sign autographs and take photos with fans. Sweet J Ekworomadu of the Harlem Globetrotters explains how she became part of the world-famous basketball team. With incredible ball handling wizardry, rim-rattling dunks, trick shots, hilarious comedy and unequaled fan interaction, this must-see event is guaranteed to entertain the whole family. Throughout their history, the Original Harlem Globetrotters have showcased their iconic talents in 122 countries and territories on six continents, often breaking. The Globetrotters are enshrined as a team and a handful of players, along with. is a wholly-owned subsidiary of Herschend Family Entertainment Corporation, the largest family-owned themed entertainment company in the U.S. Sweet j harlem globetrotters plus#With a star-studded roster featuring Big Easy Lofton, Ant Atkinson, Hi-Lite Bruton, Thunder Law, Bull Bullard, Firefly Fisher and Moose Weekes – plus female stars TNT Maddox, T-Time Brawner and Sweet J Ekworomadu* – the Globetrotters’ one-of-a-kind show is unrivaled in the world of family entertainment. Joyce 'Sweet J' Ekworomadu from the Harlem Globetrotters visited the Basketball Hall of Fame in Springfield recently. ![]() The Globetrotters will also play a portion of each game with a camouflage basketball as a sign of respect to all who protect our freedom. ![]() Fans can nominate a Harlem Globetrotters Hometown Hero at. The team is searching for active, wounded or retired members of the military who have made their community proud with their brave service and exemplary character. In addition to the highly-anticipated matchup on the court, the Globetrotters, known worldwide as the Ambassadors of Goodwill™, will honor a Harlem Globetrotters Hometown Hero at each of their 310-plus games in North America. It has been five years since the Globetrotters and Generals have faced off, as the Generals have been searching far and wide for the best players to retool their roster and have been preparing for one thing: victory over the Globetrotters. Harlem Globetrotters Legend Sweet Lou Dunbar has been both a player and coach during his storied career with the organization, and he continues to mentor as the team’s director of player personnel in charge of developing the Globetrotters’ new generation of talent as well as being a head coach for an eighth season. The Globetrotters will face a great challenge this year, as the infamous Washington Generals are more determined than ever to match the franchise’s historic last victory over the Globetrotters nearly a half century ago. Tuesday to attempt a trick shot from the top of the AtmosFEAR ride. “It’s important to stand up for one another and show others that you care about them,” Sweet J told the youth.Tickets are: COURTSIDE – $94 VIP – $61, $46, $39, $26 For tickets visit, call 1-86, or visit the USC Aiken Convocation Center Box Office. Bull Bullard and Sweet J Ekworomadu of the Harlem Globetrotters visited Morey’s Piers in Wildwood, N.J. Sweet j harlem globetrotters how to#During an interactive workshop, Sweet J talked to the Girl Scouts and Boy Scouts about how to take a stand against bullying behaviors. The program, which was designed in coordination with the National Campaign to Stop Violence, focuses on teaching children about action, bravery and compassion. ![]() Prior to the game, more than 200 Girl Scouts and Boy Scouts met with Sweet J, the 12th female member of the team, for a special program, “The ABCs of Bullying Prevention.” Saperstein renamed them the Harlem Globetrotters, even though they were from Chicago, the team arriving in a Model T Ford for their debut game on January 7, 1927. J takes a shot) Jean-Sébastien Giguère (Stanley Cup & Conn Smythe winner, hired me to perform for his son. The internationally acclaimed Harlem Globetrotters made a stop in Norfolk recently as part of their 90th anniversary tour. Sweet J is the 12th woman to play for the Globetrotters since Olympic gold-medalist Lynette Woodard broke the gender barrier in 1985. Sweet J of the Harlem Globetrotters stopped by CFWE to talk about how she went from being a school teacher to playing on the legendary team, the history of. Harlem Globetrotters (Sweet J Ekworomadu) Harlem Globetrotters (Too Tall Hall) Harlem Globetrottters. From left are Anasia Smith, Tamia Almond, Passion Arline, Aulani Jenkins, Camryn Reid and Lloyden Scott. The program was led by Sweet J, the 12th female member of the Harlem Globetrotters in the 90-year history of the team. Members of Suffolk Girl Scout Troop 944 attended The ABCs of Bullying Prevention workshop on in February at the Ted Constant Center in Norfolk. ![]() ![]() ![]() Linguisticsbelonging to an early stage in the transformational derivation of a sentence.Sport relatively far from home plate: He hit the ball into deep center field.profoundly cunning or artful: a deep and crafty scheme.having penetrating intellectual powers: a deep scholar.low in pitch, as sound, a voice, or the like: deep, sonorous tones.(of colors) dark and vivid: a deep red.immersed or submerged in or heavily covered with (fol.made with the body bent or lowered to a considerable degree: a deep bow.reaching or advancing far down: a deep dive.situated far down, in, or back: deep below the surface deep in the woods.extending or cutting far down relative to the surface of a given object: The knife made a deep scar in the table.having a specified width or number of items from front to back (often used in combination): shelves that are 10 inches deep cars lined up at the entrance gates three-deep.covered or immersed to a specified depth (often used in combination): standing knee-deep in water.having a specified dimension in depth: a tank 8 feet deep.ranging far from the earth and sun: a deep space probe.extending far in or back from the front or from an edge, surface, opening, etc., considered as the front: a deep shelf.extending far down from the top or surface: a deep well a deep valley.Idioms in deep water, in serious trouble: The company is in deep water and can barely make ends meet.ĭeep (dēp), USA pronunciation adj.Idioms in deep, involved: He was in too deep with her and had to break off their relationship.to act without enough thought of the consequences: The committee went off the deep end with the Christmas decorations.to become emotionally overwrought: She went off the deep end when she was turned down for promotion. ![]()
![]() ![]() Quantified fluorometrically before sequencing.Ĭurrently Available Amplicon Targets/Primers (recommended sets in bold) Primer Set Coverages (SILVA TestPrime, 0-2 mismatches) a Primer Targets Region(s) Forward Primer Reverse Primer Source(s) Archaea Bacteria Cyanos Eukarya mtDNA chlDNA Up to 380 (Illumina) or 190 (PacBio) samples are then pooled to make one library which is then Using the high-throughput Charm Biotech Just-a-Plate 96-well Normalization Kit. The PCR reactions from the same samples are pooled in one plate, then cleaned-up and normalized Hamilton Nimbus Select robot using Coastal Genomics Analytical Gels. PCR products are verified visually by running on a high-throughput Preparation for PacBio Sequel 2 is essentially the same, exceptįull-length 16S/18S/ITS fusion primers (PacBio adaptors + barcodes + specific regions) are used instead. Fungi) or ITS2 (Fungi only) genes with multiplexing which allows up to 380 samples to be run. Single round of PCR is done using "fusion primers" (Illumina adaptors + indices + specific regions) targeting various sub-regions of the 16S (Bacteria/Archaea), 18S Library Preparation → 16S/18S/ITS AmpliconsĪmplicon fragments are PCR-amplified from the DNA in duplicate using separate template dilutions (generally 1:1 & 1:10) using the high-fidelity Phusion+ polymerase. ![]() Integrity (generally unnecessary for PCR-only studies, but required for WGS sequencing). Quantification and quality-checks are done (via PicoGreen/Qubit and NanoDrop) to verify success. We have not yet ventured into RNA kits for metatranscriptomes, but this is something that we will beĮxamining in the near future. We have also tested various DNA kitsįor use with human urine without much success due to their low target biomass. Performance, as well as the necessary bead-beating (note that swab samples are notoriously difficult/variable in output due to low biomass). We tend to also use the QIAGEN PowerFecal DNA Kit as a general kit for swab and saliva extractions, as it has good inhibitor removal+overall There is no general consensus in the literature on choice of kits, other than to say all of themĪffect the final profiles to some degree and that bead-beating is most probably a must for difficult materials (and/or with Gram+ves and protist cysts) - we have currently evaluatedĪnd are using the QIAGEN PowerFecal DNA Kit with mouse pellets and human fecal samples the QIAGEN PowerSoil DNA Kit for soil particles and the QIAGEN PowerWaterĭNA Kit for water filters. Recommended for stool), samples must be immediately flash-frozen in liquid nitrogen after collection.ĭNA/RNA is extracted from the samples using the method/kit appropriate to the specific samples - this may also be a choice you make for your personal samples since you haveĮxperience with them, or you may wish to follow our choice extraction methods. For transcriptomics approaches for environmental, lab culture or biopsy samples (not All samples are kept frozen until extraction below. For the latter, fresh fecal pellets are collected from mice or human stool is sampled and then frozen immediately at -20☌ 80☌, preferrably dry without a storage buffers. For the former, varying volumes of water are typically collected onto filters or a few grams of soil are collected into tubes and frozen at -20☌ or Sample collection issues can be very specific to your sample type (ie: the medium which you are sampling) - many of our IMR projects so far focus on water/soil "environmental" Microbiome Helper GitHub (Wet-lab) - Specific page for the wet-lab protocols Sample Collection.Microbiome Helper GitHub - Homepage with access to all bioinformatics pipelines.Microbiome Helper: A custom and streamlined workflow for microbiome research. Microbiome Helper which outlines all the steps to follow for both the bioinformatics processing and the step-by-step wet-lab protocols (soon to be converted to Protocols.io): Literature and are summarized (primarily amplicon protocols) in the "IMR paper" here below that outlines Microbiome Helper, as well as on our GitHub site for These protocols are a synthesis of multiple sources in the current scientific Of up to 384 (380 samples + 4 PCR controls) for MiSeq or 192 (190 samples + 2 PCR controls) for Sequel 2. 400-500 bp (300+300 bp with ~100-200 bp overlap) or of longer reads on the PacBio Sequel 2. The following protocol summaries are for the generation+sequencing of WGS/metagenome libraries (Illumina NextSeq or PacBio Sequel 2) or PCR amplicons with multiple barcodes (ie: "indices") onĮither the Illumina MiSeq machine (paired-end mode) of length approx. ![]() ![]() ![]() The former issue can be resolved by just telling everyone what year they should have in mind when considering the question. My main criticisms of the game are that the questions are not only outdated (by, sometimes, more than 5 years) but they are also too focussed on the USA however, of course, this latter issue isn't really a problem because most people think highly of the United States (whether they ought to is a completely different issue that I won't debate here =P) and therefore trivia relating to it is generally still well-received or at the very least, quietly accepted. This feature of betting on answers, especially to questions that are usually interesting and diverse, is quite fun and is the main gimmick that keeps people going, especially in a party environment. Therefore if the real answer is 20, 19 (if the closest) is a winning answer, but not 20.5 or 22. The correct answer is the closest answer without going over. ![]() One key aspect of this game that gives it its kick is that you or your team mates do not need to come up with a good, or even remotely close answer to the real answer all that really needs to happen is that your team must choose from (or bet on) one of the different answers that every team has provided. ![]() This is a pretty good party/trivia game that incorporates the classic and ever popular theme of wagering. Crapuchettes) which prima facie looks extremely similar to Wits and Wagers albeit with different (and a wider variety of?) categories of questions that are apparently not focused on the United States (although I wouldn't know for sure as I've never played it).Ĩ.0 out of 10. There is also even a game called "Gambit 7" (also designed by Mr. #WITS AND WAGERS QUESTIONS LIST FREE#You are of course free to make your own rule modifications (and indeed, questions) in any event to suit. Whether any of this is true I don't know for sure but please do be wary of the different versions out there. Of course, only you would know your friends and relatives the best.Īlso, we are told that apparently the Family edition is better for kids. Whether this is so is highly questionable: plenty of "non-gamer" friends, relatives or family, including those who are academics, would enjoy that kind of environment and feel to a game. Therefore the Party edition is supposedly targeted at non-gamers (such as relatives or family) and removes the casino feel of the game. questions that are less academic or serious) whilst its gameplay involves less "accounting" in the sense that scoring is simplified (which I think obviates the need for bets to be paid out). I would label this as, a game for everyone.Also, the normal edition I am reviewing only has 100 question cards in contrast to the other two versions which have 125 cards.īoth the Family and Party editions allege that they are different to the normal version in terms of their rules, questions and gameplay.įor example, according to Dominic Crapuchettes on BoardGameGeekthe Party edition has questions that are lighter and "more fun" (ie. It was quite funny to see how far off people were on the actual correct answer. It had everyone laughing and having a good time. This can get difficult or frustrating for Canadians. The one thing I am unsure about is the fact that it is an American game and there are a lot of American based questions. ![]() It’s a great party game for all ages and all type of boardgamers. When I say simple I mean it, My 5 year old nephew was playing this game people. This game is really simple, You answer a question and and place a bet on what you think the answer is. The betting system, is one my favourite aspects, it reminds me of poker. shit! The poker chips with $2500 on them look awesome. Oh no there are lots of different colour chips. Looks intriguing, Lots of question, I think I am gonna like this game. ![]() It is compatible with macOS Big Sur, macOS Catalina (10.15), macOS Mojave (10.14), macOS High Sierra (10.13), and macOS Sierra (10.12). Out of Space: Buddy Guy w/ Todd Park Mohr of Big Head Todd & The Monsters (solo) TICKETS. 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Nautical twilight occurs when the center of the Sun is between 6 degrees and 12 degrees below the horizon. Several countries use this definition of civil twilight to make laws related to aviation, hunting, and the usage of headlights and street lamps. Only the brightest celestial objects can be observed by the naked eye during this time. There is enough natural sunlight during this period that artificial light may not be required to carry out outdoor activities. Brightest Form of TwilightĬivil twilight is the brightest form of twilight. In the evening, it begins at sunset and ends when the Sun reaches 6 degrees below the horizon.Ĭivil dawn is the moment when the center of the Sun is 6 degrees below the horizon in the morning.Ĭivil dusk is the moment when the center of the Sun is 6 degrees below the horizon in the evening. ![]() In the morning, civil twilight begins when the Sun is 6 degrees below the horizon and ends at sunrise. Business Date to Date (exclude holidays)ĭifferent degrees of Civil Twilight, Dawn, and DuskĬivil twilight occurs when the Sun is less than 6 degrees below the horizon. ![]() |
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